Sterilization of sterlet Acipenser ruthenus by using knockdown agent, antisense morpholino oligonucleotide, against dead end gene
نویسندگان
چکیده
منابع مشابه
Efficient Gene Transfer and Gene Editing in Sterlet (Acipenser ruthenus)
The sturgeon (Acipenseriformes) is an important farmed species because of its economical value. However, neither gene transfer nor gene editing techniques have been established in sturgeon for molecular breeding and gene functional study until now. In this study, we accomplished gene transfer and gene editing in sterlet (Acipenser ruthenus), which has the shortest sexual maturation period of st...
متن کاملIdentification of metallothionein gene structure in sterlet (Acipenser ruthenus)
Aquatic organisms present, not only simple sources of accumulated metal, but can interact with metals, altering their toxicity. Due to exposition of biosphere with metals, organisms have developed various defense mechanisms to protect themselves against adverse effects of these ions and their compounds. Metallothionein (MT) is one of that which represents a critical mechanism for detoxification...
متن کاملSite heteroplasmy in the mitochondrial cytochrome b gene of the sterlet sturgeon Acipenser ruthenus
Sturgeons are fish species with a complex biology. They are also characterized by complex aspects including polyploidization and easiness of hybridization. As with most of the Ponto-Caspian sturgeons, the populations of Acipenser ruthenus from the Danube have declined drastically during the last decades. This is the first report on mitochondrial point heteroplasmy in the cytochrome b gene of th...
متن کاملIdentification of metallothionein gene structure in sterlet (Acipenser ruthenus)
Aquatic organisms present, not only simple sources of accumulated metal, but can interact with metals, altering their toxicity. Due to exposition of biosphere with metals, organisms have developed various defense mechanisms to protect themselves against adverse effects of these ions and their compounds. Metallothionein (MT) is one of that which represents a critical mechanism for detoxification...
متن کاملAntisense Oligonucleotide-Mediated Transcript Knockdown in Zebrafish
Antisense oligonucleotides (ASOs) are synthetic, single-strand RNA-DNA hybrids that induce catalytic degradation of complementary cellular RNAs via RNase H. ASOs are widely used as gene knockdown reagents in tissue culture and in Xenopus and mouse model systems. To test their effectiveness in zebrafish, we targeted 20 developmental genes and compared the morphological changes with mutant and mo...
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ژورنال
عنوان ژورنال: Theriogenology
سال: 2015
ISSN: 0093-691X
DOI: 10.1016/j.theriogenology.2015.07.003